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Our Services

Zetobit serves clients in the biotech, pharma, and life sciences industry — and the academic groups that collaborate with them — delivering bioinformatics expertise across the full research pipeline. Whether you're accelerating drug discovery, building a genomics platform, or advancing a research program, we curate datasets, mine public repositories, design statistically rigorous studies, run state-of-the-art sequencing, and deliver deep analysis with compelling visualizations. Already mid-project? We plug in exactly where you need us.

Institutional-grade bioinformatics. Built for biotech, pharma, and the research teams that drive them. From data curation to publication-ready results — we plug in wherever you need us. Book a free consultation →

Research Pipeline — Plug & Play at Any Stage
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Public Dataset
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Experimental
Design
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Sequencing
Services
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Proprietary Analysis
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Step 01
Data Curation
Systematic collection, cleaning, standardization, and annotation of raw biological datasets — ensuring every downstream analysis starts from a solid foundation.
Step 02
Public Dataset Analysis
Mining and reanalysis of GEO, SRA, TCGA, ENCODE, and other repositories to extract novel insights, validate hypotheses, and reduce experimental costs.
Step 03
Experimental Design
Power analysis, cohort structuring, control selection, and protocol guidance to ensure statistically robust, reproducible, and publication-ready studies.
Step 04
Sequencing Services
State-of-the-art NGS across WGS, WES, RNA-seq, ChIP-seq, ATAC-seq, and single-cell platforms with CAP/CLIA-grade quality standards and fast turnaround.
Step 05
Proprietary Analysis & Visualization
End-to-end analysis of your proprietary datasets with publication-quality figures, interactive dashboards, and narrative reports that turn complex findings into clear decisions.
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Data Curation

Reliable analysis begins with reliable data. We specialize in end-to-end data curation — sourcing, cleaning, harmonizing, and annotating datasets from diverse origins including proprietary experimental results, electronic health records, and major public repositories such as GEO, TCGA, UK Biobank, dbGaP, and CCLE. Our team resolves inconsistencies in metadata, standardizes nomenclature across platforms and cohorts, flags and removes low-quality samples, and structures data into analysis-ready formats. The result is a curated, reproducible dataset that your team can trust — and that holds up to regulatory and peer scrutiny alike.

Starting with messy or inconsistent data? That's exactly where we begin. Tell us about your dataset and we'll outline how we'd approach it. Schedule a scoping call →

CCLE UK BioBank TCGA dbGaP
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Public Dataset Analysis

The world's leading biological databases hold answers your next experiment hasn't asked yet. We mine and reanalyze data from repositories including GEO, SRA, TCGA, UK Biobank, ENCODE, dbGaP, and CCLE to surface insights that reduce experimental costs, validate hypotheses before committing resources, and identify biomarkers, expression signatures, and population-level trends hidden in existing data. Our team builds custom pipelines tailored to each repository's data structure and quirks — normalizing across studies, correcting for batch effects, and integrating multi-omics layers to give you a richer, more complete picture. The result is faster, evidence-backed decision-making without the cost and timeline of generating new data from scratch.

Not sure if the data you need already exists? It probably does. We'll help you find it, reanalyze it, and extract what's relevant to your program — before you spend on new experiments. Talk to our team →

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Experimental Design

A poorly designed experiment cannot be rescued by even the most sophisticated analysis. Our experimental design consultations are built around your specific biological question, sample constraints, and downstream analytical goals. We advise on study architecture — including case-control frameworks, longitudinal designs, and multi-cohort strategies — and determine the statistical power needed to detect meaningful effect sizes with confidence. We guide sequencing platform and assay selection, recommend appropriate sample sizes, define inclusion and exclusion criteria, and establish quality thresholds before a single sample is processed. For multi-omics studies, we design integrated collection strategies that ensure each data layer is compatible and analytically coherent. The goal is a study that generates clean, high-confidence data from day one — and stands up to the scrutiny of both internal review and external publication.

Designing a study and want a second set of expert eyes before you commit resources? A single consultation can prevent months of rework. Let's look at your design together. Book a design review →

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Sequencing Services

We support a comprehensive range of next-generation sequencing applications — including whole genome sequencing (WGS), whole exome sequencing (WES), bulk RNA-seq, single-cell RNA-seq (scRNA-seq), ChIP-seq, ATAC-seq, and targeted panel sequencing — across human, model organism, and non-model species. Every sequencing project is preceded by rigorous library quality assessment and sample QC to ensure data integrity before sequencing begins. Raw data is processed through validated primary analysis workflows — alignment, variant calling, quantification — and delivered in analysis-ready formats alongside comprehensive QC reports. Whether you are sequencing a handful of discovery samples or scaling to a large clinical or population cohort, our workflows are designed to maintain consistency, reproducibility, and high data quality at every scale.

Have samples ready to sequence — or data already in hand? We support projects at any stage, from library prep through final variant report, with rigorous quality standards throughout. Get a project estimate →

Sequencing
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Proprietary Dataset Analysis & Visualization

Your proprietary data contains answers that generic pipelines will miss. We deliver end-to-end analysis of your internal datasets — whether derived from sequencing, proteomics, metabolomics, or multi-omics experiments — using analytical frameworks tailored to your specific biological question and program stage. Our team performs differential expression analysis, variant annotation and interpretation, pathway and gene ontology enrichment, survival analysis, dimensionality reduction, and single-cell deconvolution, among other methods. Where relevant, we integrate your proprietary data with curated public datasets to provide comparative context, benchmark your findings against existing literature, and surface novel associations that internal data alone cannot reveal. All findings are delivered as publication-quality figures, interactive visual reports, and clear written interpretations designed for both scientific and non-scientific stakeholders — from your bench scientists to your leadership team.

Sitting on proprietary data that hasn't told its full story yet? We turn complex multi-omics datasets into findings your scientific and leadership teams can act on. Start with a free consultation →

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Our Team

Kanna Nandakumar

Kanna Nandakumar, PhD

Founder and CEO

Dr. Nandakumar is a computational biologist and clinical bioinformatics specialist with over 14 years of experience advancing genomic medicine across research, translational, and clinical settings. Prior to founding Zetobit, he served as the principal architect of the Variant Interpretation Platform (VIP) at the University of Kentucky — a CAP/CLIA-compliant bioinformatics infrastructure supporting NGS-based oncology panels, constitutional disorder diagnostics, and medical exome interpretation. His technical expertise encompasses whole genome sequencing, RNA sequencing, targeted panel design, and multi-omics data integration, underpinned by a rigorous command of the regulatory frameworks governing clinical genomic applications. Dr. Nandakumar founded Zetobit LLC to deliver institutional-grade bioinformatics solutions to biotechnology, pharmaceutical, and academic medical clients. His work is defined by a commitment to scientific rigor, analytical precision, and the translation of complex genomic data into clinically and commercially actionable insights.

LinkedIn Google Scholar
Justin Duplantis

Justin Duplantis, MBA

Chief Business Officer

Mr. Duplantis brings over two decades of sales leadership and business development experience to Zetobit, with a focused specialization in the life sciences industry. His academic foundation in Chemistry (B.S.) and Data Analytics (MBA) positions him at the intersection of scientific credibility and commercial strategy — enabling him to engage clients, institutional partners, and technology stakeholders with equal fluency. Throughout his career, Mr. Duplantis has built and led high-performing commercial teams, established strategic partnerships with research institutions, sequencing providers, and industry organizations, and driven sustained revenue growth across complex, technically demanding markets. At Zetobit, he leads all business development and client engagement functions, working to align the company's bioinformatics capabilities with the specific research and operational objectives of each client. His approach prioritizes long-term partnership over transactional engagement — connecting organizations with solutions that meaningfully advance their scientific and commercial goals.

LinkedIn
Bioinformatics Team

Bioinformatics Team

Global Experts

Zetobit's bioinformatics team is a globally distributed network of specialists with deep expertise across genomics, transcriptomics, proteomics, and computational biology. Each engagement is staffed to match the precise technical demands of the project — drawing on domain-specific experience in clinical pipeline development, multi-omics integration, statistical modeling, and data visualization. Our team operates within a broader scientific partner ecosystem encompassing leading research institutions, technology providers, and subject matter experts, enabling Zetobit to extend its capabilities fluidly across disciplines and scales of complexity. From experimental design and analytical framework development through to results interpretation and reporting, our bioinformaticians work as embedded technical partners — bringing the rigor of institutional research to the pace and precision that biotechnology and pharmaceutical clients require.

Every Zetobit engagement is led by senior bioinformatics expertise. You'll always have a direct line to the scientists making analytical decisions on your project — no matter the team composition behind it.

Book a call with our team →

Publications

GWAS

GWAS
Nature Genetics Genome-wide meta-analysis identifies 56 bone mineral density loci and reveals 14 loci associated with risk of fracture. Nature genetics 44 (5), 491.
GWAS
Acta Neuropathologica ABCC9 gene polymorphism is associated with hippocampal sclerosis of aging pathology. Acta neuropathologica 127 (6), 825-843.
GWAS
Human Molecular Genetics Large common deletions associate with mortality at old age. Human molecular genetics 20 (21), 4290-4296.
GWAS
Journal of Medical Genetics A genome-wide copy number association study of osteoporotic fractures points to the 6p25. 1 locus. Journal of medical genetics, jmedgenet-2013-102064.
GWAS
Human Molecular Genetics Targeted sequencing of genome wide significant loci associated with bone mineral density (BMD) reveals significant novel and rare variants: the Cohorts for Heart and Aging. Human molecular genetics 25 (23), 5234-5243.
GWAS
BMC Proceedings On family-based genome-wide association studies with large pedigrees: observations and recommendations. BMC proceedings 8 (1), S26.
GWAS
Springer Mihajlovic, A., Machine Learning-Based Imputation of Missing SNP Genotypes in SNP Genotype Arrays, ISBN 9781461487852, 2013, Computational Medicine in Data Mining and Modeling, Springer New York
GWAS
AACR Prevalence of pathogenic mutations in cancer predisposition genes among pancreatic cancer patients. Cancer Epidemiology and Prevention Biomarkers.

R&D NGS

R&D NGS
Cancer Research The association of glioma germline risk SNPs with mutation-based molecular subgroups. Cancer Research 74 (19 Supplement), 4714-4714.
R&D NGS
bioRxiv Clarke, D, et. al., Playbook Workflow Builder: Interactive Construction of Bioinformatics Workflows from a Network of Microservices, 2024, Cold Spring Harbor Laboratory.

Clinical NGS

Clinical NGS
Cancer Research Lau, J.W., Lehnert E., et. al., The Cancer Genomics Cloud: Collaborative, Reproducible, and Democratized—A New Paradigm in Large-Scale Computational Research, ISSN 1538-7445, DOI 10.1158/0008-5472.can-17-0387, 2017, Cancer Research, American Association for Cancer Research (AACR)
Clinical NGS
IEEE Constellation: A secure self-optimizing framework for genomic processing. e-Health Networking, Applications and Services (Healthcom), 2016 IEEE 18th ...
Clinical NGS
IPT Mihajlovic A., AI and its Involvement in the Future of Neoantigen Discovery, 2020, Innovations in Pharmaceutical Technology
Clinical NGS
Bioinformatics Bioinformatics Strategies for Identifying Regions of Epigenetic Deregulation Associated with Aberrant Transcript Splicing and RNA-editing. Bioinformatics, 163-170.

Bulk RNA-seq

Bulk RNA-seq
Nature Scientific Reports Leong P, Mihajlović A, Xi L, et. Al., Reconstructing and counting genomic fragments through tagmentation-based haploid phasing, volume 11, ISSN=2045-2322, September 2021, Nature Scientific Reports, Springer Science and Business Media LLC

scRNA-seq

scRNA-seq
Bio-Protocol Long P, Mihajlovic A., Xi L, Preparing Single-cell DNA Library Using Nextera for Detection of CNV, ISSN 2331-8325, DOI 10.21769/bioprotoc.3175, 2019, BIO-PROTOCOL

Our expertise is peer-reviewed — and available to your program. The same rigor behind these publications goes into every Zetobit engagement.

See how we can support your research →

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Zetobit, LLC

Bioinformatics Consulting

Location
710 E Main St
Lexington, KY 40502
Contact

Prefer to reach out async first? Send us a brief description of your project — we'll respond within one business day.

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© 2026 Zetobit, LLC. All rights reserved. Lexington, KY  ·  Genomics  ·  Transcriptomics  ·  Proteomics
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